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CAS NO.643-79-8
High Sensitivity
O-Phthalaldehyde (OPA) reacts with amino groups in amino acids, peptides, and proteins to form a highly fluorescent product, enabling the detection of target compounds at very low concentrations with exceptional sensitivity.
Excellent Fluorescent Properties
OPA exhibits strong fluorescence emission, especially under UV light, producing a distinct fluorescent signal, making it an ideal reagent for the quantitative analysis of amino acids, peptides, and proteins.
Wide Applicability
OPA is widely used in various analytical techniques such as High-Performance Liquid Chromatography (HPLC), Capillary Electrophoresis (CE), and Thin-Layer Chromatography (TLC) for the quantitative analysis of amino acids, peptides, and proteins.
Easy to Use
When used as a reagent for analysis, OPA typically requires minimal sample preparation. Its reaction directly produces a fluorescent signal, simplifying the experimental process, saving time and costs.
High Specificity
OPA reacts specifically with free amino groups in amino acids, providing high specificity in complex biological samples, ensuring accurate identification and analysis of target molecules while minimizing background interference.
Good Stability
Under appropriate storage conditions, OPA demonstrates excellent chemical stability, ensuring long-lasting effectiveness and reliability for extended use.
Chemical Name: O-Phthalaldehyde (OPA)
CAS Number: 643-79-8
Molecular Formula: C?H?O?
Molecular Weight: 134.1 g/mol
Appearance:Yellow crystalline powder
Solubility: O-Phthalaldehyde is soluble in organic solvents such as methanol, ethanol, and acetone, but sparingly soluble in water.
Purity: Commercial purity is typically 98% or higher.
Applications:
Amino Acid Analysis: OPA reacts with free amino groups in amino acids, producing a highly fluorescent product, widely used in quantitative amino acid analysis.
Biochemical Research: OPA is used for protein and peptide analysis and quantification.
High-Performance Liquid Chromatography (HPLC): OPA is used as a fluorescent reagent in HPLC to detect amino acids, peptides, and proteins.
Fluorescent Labeling: OPA can be used for the fluorescent labeling of biomolecules like proteins and nucleic acids, making it useful in biological and medical research as a molecular probe.
Storage Conditions: Store in a cool, dry place, away from light. Avoid contact with strong acids or bases to ensure stability.